DNP Powder
English name: 2,4-Dinitrophenol
English Name: 2,4-dinitrophenol moistened with water (H (2) O ~ 20%); 2,4-dinitrophenol, reagent grade; 2,4-dinitrophenol, industry grade; 2,4-dinitrophenolate
CAS: 51-28-5
EINECS Number: 200-087-7
Formula: C6H3N2O5
MW: 183.099
Molecular Structure: 
Melting point: 106-112 ℃
Boiling point: 312.1 ° C at 760 mmHg
Flash Point: 142.8 ° C
Water-soluble: 0.6 g / 100 mL (18 ℃)
Vapor Pressure: 0.000294mmHg at 25 ° C
Melting point 106-112 ° C
Water-soluble 0.6 g / 100 mL (18 ° C)
Uses:Used in the manufacture of dyes, picric acid and imaging agents


DNP, its role is to make the de-coupling of oxidation and phosphorylation. Once given to 10-4M concentration of mitochondria, the uncoupling effect occurs, oxygen absorption is increased, P: O decline, if further given the high concentration will hinder the absorption of oxygen. DNP as a mechanism of action uncoupler is: DNP did not inhibit transfer of electrons, but it can eliminate the proton driving force required for the synthesis of ATP. Since DNP is a lipophilic compound is weakly acidic, it passes through the neutral state proton mitochondrial inner membrane lipid bilayer. When there is a proton gradient across the membrane, it is incorporated in the acidic side of the membrane proton to become a neutral state uncharged, by diffusion through the membrane, and in the alkaline side of the membrane to release a proton, thereby neutralized cross membrane proton gradient.